Figure 5. NF-κB causes strong induction of endogenous IκBα but fails to up-regulate endogenous IκBβ gene expression in HEK-293 cells.

HEK-293 cells were transfected with a plasmid expressing a p65–RFP fusion protein or empty RFP vector. Cell lysates were generated after 24 h and subjected to SDS/PAGE and Western immunoblotting to measure levels of p65, IκBα, IκBβ, IκBϵ and β-actin. These results are representative of three independent experiments.