Table 1. Properties of PGXA, PGXB, PGXC and exo-PG.
Specific activity of the enzymes were determined towards 0.25% (w/v) PGA and 0.25% (w/v) XGA in 50 mM NaOAc (pH 4.0). The optimum pH for each enzyme was determined towards 0.25% (w/v) PGA in McIlvaine buffers (over a pH range of 2.5–7.5). n.d., not determined.
| Specific activity (units/mg) | |||||
|---|---|---|---|---|---|
| Enzyme | Molecular mass (kDa) | Calculated molecular mass (kDa) | PGA | XGA | pH optimum towards PGA |
| PGXA | 78 | 47 | 7 | 25 | 3.5–4.0 |
| PGXB | 67 | 48 | 242 | 33 | 4.0–4.5 |
| PGXC | 79 | 45 | 223 | 202 | 3.5–4.0 |
| Exo-PG | 781 | 47 | 230 | 17 | 4.21 |
| RGXB | 82 | 50 | n.d. | n.d. | n.d. |
1 As determined by Kester et al. [42].