Figure 3. Subcellular localization of CHMP7.

HeLa cells were co-transfected with plasmids encoding FLAG–CHMP7 and GFP (A–C), CHMP4b–GFP (D–F), CHMP6–GFP (G–I) or GFP–Vps4B^E235Q (J–L). After 24 h, the cells were fixed and subjected to immunofluorescence confocal microscopic analysis by direct visualization of GFP or GFP-fusion proteins (A, D, G and J; green) and by staining with anti-FLAG mAb (M2) and Cy3-labelled goat anti-mouse IgG antibody (B, E, H and K; red). Their merged images are shown in (C, F, I and L) respectively. Scale bars, 10 μm.