Figure 3. EFV, TMC120, and TMC125 Enhance Intracellular Gag and Gag-Pol Processing.

293T cells were transfected with pDRNL and treated with 5 μM of each drug. After 36 h post-transfection, cell lysates were harvested and viral proteins were detected by quantitative Western blot analysis using (A) anti-RT and (C) anti-CA antibodies. AlexFluor680 goat anti-mouse was used as the secondary antibody. Western blots were scanned on an Odyssey Infrared Imager and analyzed using Odyssey software. Quantitation of the intracellular ratio of (B) p51 to p66 and (D) p24 to Pr55^Gag. The ratios were calculated from at least three independent experiments and are expressed as the mean ± standard error.