Figure 6. Potent NNRTIs Inhibit Viral Particle Production.

Viral particle production in drug-treated 293T cells (A) and in HeLa cells (C). Top panels represent virion-associated p24 and bottom panels are p24 present in cell lysates. Cells were transfected with pDRNL and treated with drug as described in Figure 3, except that after 36 h, cells were metabolically labeled with Trans[³⁵S]-label for 4 h. Cell lysates were immunoprecipitated using anti-CA mAb and viral particles in the supernatant were pelleted through a sucrose cushion. The proteins were separated by SDS-PAGE and visualized and quantified using a phosphorimager. Viral particle production in 293T cells (B) and HeLa cells (D) was quantified as described in Materials and Methods. Data represent at least three independent experiments and are expressed as the mean ± standard error.