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. 2006 Nov 10;103(47):17967–17972. doi: 10.1073/pnas.0605461103

Fig. 6.

Fig. 6.

Neuronal expression and activity of LRRTM3. (A and B) Semiquantitative PCR of cDNAs from 48 human tissues with gene-specific primers for LRRTM3 (A) or mouse brain regions (B). (C) In situ hybridization with antisense probes to LRRTM3 in adult mouse brain (coronal section with standard anatomical markers). Ctx, cortex; Hip, hippocampus; DG, dentate gyrus; AMY, amygdala; Hyp, hypothalamus; Hb, habenular. (D) Mouse cortical primary neurons at day 5, transfected with eGFP and indicated siRNA to illustrate transfection efficiency and allow visualization of morphology. (E Left) Mean Aβ40 from conditioned medium of mouse primary neurons transfected with wild-type APP ± BACE1 siRNAs. (E Right) Mean Aβ40 levels normalized to nonsilencing (NS) controls for primary neurons cotransfected with cDNA encoding wild-type APP plus BACE1 or LRRTM3 siRNAs. (F) Standard (Lower) and confocal (Upper) immunofluorescence images of SH-SY5Y cells cotransfected with APP and LRRTM3-FLAG cDNAs. The right image is merged. ∗, P < 0.05 ANOVA. Error bars represent the standard deviation. (Scale bar, 20 μM.)