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. 2006 Nov 10;103(47):17801–17806. doi: 10.1073/pnas.0608484103

Fig. 2.

Fig. 2.

ELYS localizes to the nuclear pore in interphase and to kinetochores during mitosis. (A) Double immunofluorescence on permeabilized, then fixed, HeLa cells with anti-ELYS antibody (Top, green) and the anti-FG Nup monoclonal antibody, mAb414 (Middle, red), revealing that both localize to the nuclear rim in a punctate pattern characteristic of nuclear pores. Superposition of the signals (Bottom) and a 250-fold magnification (see Insets to the right) show that ELYS colocalizes with the FG Nups. The nuclear (n) and cytoplasmic (c) sides of the nuclear envelope are indicated. (B) Immunofluorescence on mitotic HeLa cells extracted with PHEM buffer. Insets show a 250-fold magnification of kinetochore stains. ELYS (left column, green) and Nup133 (right column, green) show similar localization and bracket CENP-B (red) on the kinetochores. The DNA is stained with DAPI (blue). (C) During nuclear assembly in HeLa cells, ELYS (left column and red) associates with the chromatin periphery in late anaphase, whereas Nup62 (center column and green) associates in telophase. (Scale bars: A and B, 5 μm; C, 10 μm.)