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CD and HSQC characterization of Nogo-66 fragments. (a and b) Far-UV CD and NMR HSQC spectra of Nogo-66; (c and d) Nogo-60; and (e and f) Nogo-(21-60). All three peptides were only soluble in salt-free water (pH 4.0), with protein concentrations of ∼100 μM for Nogo-66, and ∼200 μM for Nogo-60 and Nogo-(21-60). NMR spectra were acquired on a Bruker Avance 800 MHz NMR spectrometer at 20°C.
