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Spinning disk FCS applied to microspheres diffusing in (homogeneous) water-glycerin. The autocorrelation curves for 16 different pixels are plotted in panel a, and panel b shows their average, along with best fit curve to Eq. 11 with α, is fixed at 1. (c) 128 × 128 maps of the mean concentration 〈N〉 and (d) diffusion coefficient (in μm2/s). The pixel size is 178 nm. The variation is due primarily to statistical noise, but also deviant large particles (or clumps).
