Expression pattern of Tbx5 in Xenopus tropicalis. The expression pattern of Tbx5 detected by in situ hybridization between late neurula and early tadpole stages is shown (uncleared except for g). a–j; Stages are as follows: stage 19 (a,b), stage 25 (c,d), stage 26 (e,f), stage 31/32 (g; cleared), stage 33 (h), and stage 40 (i,j). Except for the anterior view in b and the ventral view in j, embryos are oriented with anterior to the left. Anterior is to the top in j. ea, eye anlagen, hp, heart primordium, v, ventricle.