Figure 2.

Interactions between peptide appendices of human ARSs determined by the yeast two-hybrid system. The peptide appendices of human ARSs were expressed as LexA and B42 fusion proteins in yeast. The positive interactions were determined by cell growth on leucine-depleted medium (Left), as well as by the formation of blue colonies on medium containing X-Gal (Right). Cells were incubated for 3 days at 30°C. In the cases of EPRS and IRS, only single copies (EPRS-R3 and IRS-R2) of their repeated sequences were used for the experiment (see Fig. 1). N and C indicate the N- and C-terminal extensions of the complex-forming ARSs, respectively.