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. Author manuscript; available in PMC: 2006 Nov 13.
Published in final edited form as: Pigment Cell Res. 2005 Dec;18(6):417–426. doi: 10.1111/j.1600-0749.2005.00265.x

Figure 4.

Figure 4

Immunofluorescence of (A and B) normal and (C–F) HPS-1 melanocytes immunolabeled for tyrosinase (green in A and C) or Tyrp1 (green in E and F) and ribophorin II (red) (B and D represent images A and C in which the green channel was removed during photo reproduction. All images were captured from the confocal microscope using identical settings for each primary antibody.) Minimal expression of ribophorin II occurred in the perinuclear area (arrowheads) of normal melanocytes where significantly more expression with a more prominent reticular pattern was apparent in HPS-1 melanocytes (arrowheads). Higher magnification of co-labeled HPS-1 melanocytes (E and F) demonstrating minimal co-localization of Tyrp1 with ribophorin II at peripheral edges of macroautophagosomes (arrows). On rare occasions, extensive co-localization of Tyrp1 and ribophorin II was observed in a macroautophagosome (arrow with asterisk). N, nucleus; Bar, 20.0 μm.