Skip to main content
. Author manuscript; available in PMC: 2006 Nov 13.
Published in final edited form as: Pigment Cell Res. 2005 Dec;18(6):417–426. doi: 10.1111/j.1600-0749.2005.00265.x

Figure 8.

Figure 8

Ultrastructural analysis of early events in the trafficking of exogenously gold-labeled horseradish peroxidase (HRP-Au18). HPS-1 melanocytes were incubated in HRP-Au18 for 6 h, chased for either 2 or 4 h, and then processed for electron microscopy as described in Materials and methods. After a 2 h chase (A and C), HRP-Au18 could be found associated with the plasma membrane (black arrowheads), small early endosome-like compartments in the periphery of the cell (white arrowheads), and the vicinity of macroautophagosomes (black arrowhead with asterisk). In contrast, HRP-Au18 could not be found within the macroautophagosomes (arrows). After a 4 h chase (B and D), HRP-Au18 could be found in larger endosome-like compartments (black arrowheads) prominent in the Golgi area (G) and within the macroautophagosomes (arrow). Bars = 0.5 μm.