Table III.
Farm number | Strain | Serotypea | Origin | Used in autogenous vaccine? | PFGE pattern | RsaI pattern | MboII pattern | Combined patternb |
---|---|---|---|---|---|---|---|---|
1 | 347 | 2 | T | No | P3 | R31 | M15 | C2 |
348 | 1/2 | T | No | P3 | R31 | M15 | C2 | |
349 | 2 | T | No | P3 | R31 | M15 | C2 | |
350 | 2 | T | No | P3 | R31 | M15 | C2 | |
351 | NT | E | No | P5 | R43 | M6 | C3 | |
352 | NT | E | No | P5 | R43 | M6 | C3 | |
2 | 353 | 1/2 | T | No | P1 | R24 | M8 | C1 |
3 | 311 | 2 | S | No | P2 | R27 | M10 | C4 |
330 | 2 | S | No | P1 | R24 | M8 | C1 | |
336 | 2 | S | No | P1 | R28 | M8 | C5 | |
337 | 2 | S | No | P1 | R24 | M7 | C6 | |
338 | 2 | S | No | P1 | R24 | M8 | C1 | |
340 | 2 | S | Yes | P1 | R24 | M8 | C1 | |
341 | 2 | S | Yes | P1 | R24 | M8 | C1 | |
354 | 2 | T | No | P1 | R24 | M8 | C1 | |
4 | 355 | 1/2 | T | No | P1 | R10 | M8 | C7 |
5 | 289 | 2 | S | No | P1 | R24 | M8 | C1 |
326 | 2 | S | No | P1 | R24 | M8 | C1 | |
331c | 2 | S | No | P1 | R24 | M8 | C1 | |
332c | 2 | S | No | P1 | R24 | M8 | C1 | |
342 | 2 | S | Yes | P1 | R38 | M10 | C8 | |
356 | 2 | T | No | P1 | R24 | M8 | C1 | |
357 | 2 | T | No | P1 | R5 | M8 | C9 | |
358 | 2 | T | No | P1 | R16 | M8 | C10 | |
359 | 2 | T | No | P1 | R24 | M8 | C1 | |
360 | 2 | T | No | P1 | R24 | M8 | C1 | |
361 | 2 | T | No | P1 | R24 | M8 | C1 | |
362 | 2 | T | No | P1 | R24 | M8 | C1 | |
6 | 320 | 2 | S | No | P1 | R24 | M8 | C1 |
345 | 2 | S | Yes | P1 | R24 | M8 | C1 | |
7 | 288 | 2 | S | No | P2 | R38 | M10 | C11 |
343 | 2 | S | Yes | P2 | R10 | M11 | C12 | |
344 | 2 | S | Yes | P1 | R24 | M8 | C1 | |
363 | 2 | T | No | P1 | R24 | M8 | C1 | |
8 | 297 | 2 | S | No | P1 | R24 | M8 | C1 |
346 | 2 | S | Yes | P1 | R24 | M8 | C1 |
PFGE — pulsed-field gel electrophoresis; T — tonsil; NT — nontypable; E — environment; S — septicemia.
Strains 331 and 332, as well as the tonsil and environment isolates, were collected in the current study; the others strains were collected from earlier outbreaks.
Obtained from the combined results with PFGE and ISR-RFLP (an approach based on PCR amplification of a fragment of rRNA genes, including a part of the 16S and 23S genes and the 16S–23S rDNA intergenic spacer region [ISR], followed by restriction fragment length polymorphism).
These strains were isolated from blood samples of 2 pigs that died suddenly on the day of the visit to farm 5.