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. 2007 Jan;71(1):34–40.

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Detection of abnormal prion protein (PrP^Sc) in animals with scrapie by a sensitive Western blot procedure involving enrichment by precipitation with sodium phosphotungstic acid (PTA) before and after composting. Lanes 1 to 3 are the results for bag 3 from sheep 1 in experiment 1: PrP^Sc was detected on day 0 (lane 1) but not after composting in tissue remnants (lane 2) or immediately surrounding sawdust (lane 3). The results for the other bags in experiment 1 were similar. Lanes 4 to 7 are the results for bag 5 of experiment 2: PrP^Sc was detected on day 0 in both the PTA-enriched sample, which had an excessive amount of PrP^Sc loaded onto the gel (lane 4), and the crude extract (lane 5) but not after composting in tissue remnants (lane 6) or immediately surrounding sawdust (lane 7), both PTA-enriched. Lanes 8 to 10 are the results for bag 2 of experiment 2: PrP^Sc was detected on day 0 in both the PTA-enriched sample (lane 8) and the crude extract (lane 9), as well as after composting in PTA-enriched tissue remnants (lane 10).