Table II.
Primers used for microbial DNA analysis
| Primer (and reference) | Nucleotide sequence (5′–3′)a | Positionb | Target region of 16S ribosomal DNA |
|---|---|---|---|
| 8f (11) | CAC GGA TCC AGA GTT TGA T (C/T) (A/C) TGG CTC AG | 8 | Entire 16S rDNA |
| 1510r (11) | GTG AAG CTT ACG G(C/T)T ACC TTG TTA CGA CTT | 1510 | Entire 16S rDNA |
| Com1 (12) | CAG CAG CCG CGG TAA TAC | 519 | V4–V5 |
| Com2-GC (12) | CGCCCGGGGCGCGCCCCGGGCGGGGCGGGGGCACGGGGGG CCG TCA ATT CCT TTG AGT TT | 907 | V4–V5 |
a
The underlined portion indicates GC-clamp, a DNA sequence rich in guanine and cytosine that is attached to the primer to avoid complete strand separation for detection of all possible base substitutions in the target fragment using denaturing-gradient gel.
b
Based on the sequence in Escherichia coli.