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. 2006 Aug 25;72(11):6948–6954. doi: 10.1128/AEM.00976-06

FIG. 3.

FIG. 3.

Synthesis of Cry11A and Cyt1A using the minireplicon sequence derived from pBtoxis. (A) Map of recombinant plasmid pBtH constructed using the minireplicon in pBtSw (Fig. 1) and the ∼10-kb HindIII fragment from pBtoxis that contains the cry11A, cyt1A, and 20-kDa genes. (B) Phase-contrast microscopy showing crystals (arrows) in cells of B. thuringiensis subsp. israelensis strain 4Q7 transformed with pBtH. (C) SDS-PAGE analyses of recombinant strains and wild-type strains of B. thuringiensis subsp. israelensis, strain 4Q7 pBtH (lane 1), strain 4Q5 (lane 2), strain pBtSw (lane 3) (Fig. 1), and strain 4Q7 (lane 4). Lane M, protein molecular mass standards.