TABLE 3.
Identification of the major bacterial flora of two commercial cheeses by 16S rRNA gene sequencinga
| Representative clone | Colony morphotype
|
Colony count (CFU/g) | GenBank accession no. | Closest phylogenic affiliation (GenBank accession no.) | % Identity | |
|---|---|---|---|---|---|---|
| Color | Diam (mm) | |||||
| Cheese 1 | ||||||
| 1MA | Slightly orange | 5 | 2.0 × 109 | DQ361013 | C. casei DPC 5300 (AF267155) | 99 |
| 1MB | Cream | 5 | 1.3 × 109 | DQ361014 | C. casei DPC 5300 (AF267155) | 99 |
| 1MC | Yellow | 5 | 1.2 × 109 | DQ361012 | A. arilaitensis Po102 (AJ609627) | 100 |
| 1MD | Grayish white, semitranslucent | 3 | 8.0 × 108 | DQ361015 | M. psychrotolerans INCT119 (AB159717) | 100 |
| 1ME | White with irregular edge | 8 | 6.7 × 107 | DQ361017 | S. xylosus ATCC 29971 (D83374) | 100 |
| 1MF | Orange | 2 | 3.3 × 107 | DQ361016 | B. linens SB1 (AJ315491) | 99 |
| Cheese 8 | ||||||
| 8MA | Slightly cream | 5 | 3.1 × 109 | DQ361022 | C. casei DPC 5300 (AF267155) | 99 |
| 8MB | Yellow | 5 | 1.3 × 109 | DQ361019 | A. arilaitensisPo102 (AJ609627) | 100 |
| 8MC | Cream | 5 | 1.1 × 109 | DQ361021 | C. casei DPC 5300 (AF267155) | 99 |
| 8MD | Slightly orange | 8 | 1.1 × 109 | DQ361018 | A. arilaitensis Po102 (AJ609627) | 100 |
| 8ME | Greyish white | 3 | 4.0 × 108 | DQ361023 | A. arilaitensisPo102 (AJ609627) | 100 |
| 8MF | Slightly yellow | 5 | 2.3 × 108 | DQ361020 | A. arilaitensis Po102 (AJ609627) | 100 |
a
For each cheese sample, the different types of colonies grown on BHI agar were enumerated, and the 16S rRNA gene of one representative clone of each morphotype was then sequenced.