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Production of RpoS by V. cholerae in low-phosphate medium. Strains C6709 (wild type) and AJB37 (ppk) were grown to stationary phase in MOPS-G medium containing 2 mM phosphate. Cells were centrifuged and resuspended in 1 volume of MOPS-G medium containing 0.1 mM phosphate. Cultures were incubated at 37°C, and samples were taken at 2 and 4 h and used for Western blot analysis.
