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ATP biosynthesis in V. cholerae ppk mutants. Strains C6709 (wild type) and AJB37 (ppk) were grown to stationary phase in MOPS-G medium containing 2 mM phosphate. Cells were centrifuged and resuspended in 1 volume of MOPS-G medium containing 2 mM phosphate (open bars) or 0.1 mM phosphate (solid bars). Light production and the number of CFU were determined as described in Materials and Methods. The error bars indicate the standard deviations of the means for three independent cultures.
