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. 2006 Aug 25;72(11):7324–7330. doi: 10.1128/AEM.01163-06

FIG. 4.

FIG. 4.

Development of a mushroom structure in an S. oneidensis biofilm with a ribosomal (growth) reporter (DKN310) (A to E) and an anaerobic reporter gene (mtrB) (DKN312) (F to J). The grid squares are 10 μm by 10 μm. In the first column the cells are constitutively expressing ecfp and the fluorescence from ecfp is false red. In the second column of panels A to E, the cells expressing the growth-active GFP(AAV) are green. In the second column of panels F to J, the cells expressing the mtrB reporter are green. The third column is an overlay of the red and green channels. (A) 18 h and 8 μm high; (B) 28 h and 18 μm high; (C) 41 h and 52 μm high; (D) 65 h and 92 μm high; (E) 77 h and 112 μm high; (F) 17 h and 8 μm high; (G) 29 h and 31 μm high; (H) 47 h and 58 μm high; (I) 71 h and 104 μm high; (J) 85 h and 118 μm high.