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. 2006 Aug 25;72(11):6980–6985. doi: 10.1128/AEM.01128-06

TABLE 1.

Primers used for RT-PCR analysis of P. carbinolicus cytochromes and cytochrome cbiosynthesis genes

Gene Description Forward primer (5′-3′) Reverse primer (5′-3′) Annealing temp for PCR (oC)
Cytochromes
    PCAR2944 Glutamate synthase, large subunit CAGCATGCCATCAAGTTCGT ATAATGCGTACATCGGCCG 60
    PCAR2570 Cytochrome c family protein TTCGCTATCTCCCTCGTTCAA TGGCCAAAGTACGGACAATG 60
    PCAR2549 Hypothetical protein CGGCTTGCTGTTTGGTACTGT TGAACGGCATCGAATGGTTAC 60
    PCAR2529 caa3-type cytochrome coxidase, subunit II GAGGCGGTGGACAAGGTTTT GGAAGGCCGGGATATAAAGG 60
    PCAR0558 Cytochrome c family protein CTTGACGGTGATGGCATGC CGCAAGGATATGTTCAGCCAC 60
    PCAR0192 Molybdopterin oxidoreductase/precorrin-4 methylase CTGGCGAAAATCCTTGAACAA AGGTAGGTGGCAAAGCGCT 60
    PCAR2867 Cytochrome c nitrite reductase, nrfH ACTTCAGTACGGACCCGACG CCCGTTGTCATGAACTCTTCC 60
    PCAR2866 Cytochrome c nitrite reductase nrfA TCGAGAAGACCTGGGATGAGA ACTTCAGTGCGGCAATTTCC 60
    PCAR2550 Cytochrome c CACGGTTCCCAAAAATTCCA CCATCCCATTTTCAACGAGC 60
    PCAR1628 Cytochrome c7, ppcA CTGTTCCGACGCCATATCAA GCAACCACCGCAGTCTGTC 60
    PCAR0152 Hypothetical protein GGTACCGGCATCGCTTTTC CATCAAACAACCGGGCATC 60
    PCAR2984 Cytochrome c family protein ATGAAAAAATGCCTTTGGATGCT GCAACGGAAGCAGGGTTCT 60
    PCAR2745 Hypothetical protein TGGTGGCGGATTTTCTTCA TGCAGAACATCTTCCCGGATA 60
    PCAR2069 Protein-disulfide isomerase AACAGGCCGACAAGGTTTTG GCGGTTACAGACGATGCTCTT 60
Heme biosynthesis
    PCAR3065 ccsA homolog GCGCTGGTGCTGATGATTTT TCCCGAAAGCAGCAGATTG 55
    PCAR3064 Glutamyl tRNA synthetase, hemA GTCCTATGCGGCGGTGG AAACGCACGATTTCCTGCTC 55
    PCAR0266 Glutamate 1-semialdehyde aminotransferase, hemL CAGCCCTGTTCGTGCATTTA ACCCTGCCCAGATCGTTGT 55
    PCAR0770 Ferrochetalase, hemH GGCCGTGATTATGCGCTACT ACGCCCTCATCCACCAAG 55
Cytochrome c biogenesis
    PCAR2229 Heme transport and ligation, ccsB GCCTGCGTTCCCTGAAACT CGCCGAGCAATATGATCAAA 55
    PCAR2228 Heme transport and ligation, ccsA GATTGCGTCGCGAAGGG AATTGACATCATCCAGCGTGG 55
    PCAR1954 Thioredoxin, resA TTTCTGGTGCTGTCTGTCGG GCGCAATGCTCCGGC 55
    PCAR1953 Thioredoxin, ccdA GCCCTGTGTATTGCCGCT GAGAGCAAAAACGGCAATCC 55