TABLE 2.
Predicted c-type cytochromes in P. carbinolicus and mRNA expression determined by RT-PCR
| Gene | Annotation | No. of CXXCH motifs | Associated gene(s) | Function | mRNA expression
|
|
|---|---|---|---|---|---|---|
| Acetoin | Fe(III) NTA-ethanol | |||||
| Cytoplasmic membrane associated | ||||||
| PCAR2944 | Glutamate synthase, large subunit | 1 | None | Unknown | − | + |
| PCAR2570 | Cytochrome c family protein | 1 | PCAR2571 | In operon with another membrane-bound protein | − | − |
| PCAR2549 | Hypothetical protein | 1 | PCAR2550 to PCAR2553 | In operon with quinol:cytochrome coxidoreductase | + | + |
| PCAR2529 | caa3-type cytochrome c oxidase, subunit II | 1 | PCAR2526 to PCAR2528 | Terminal oxidase | + | + |
| Soluble periplasmic | ||||||
| PCAR0558 | Cytochrome c family protein | 1 | None | Unknown | + | + |
| PCAR0192 | Molybdopterin oxidoreductase/precorrin-4 methylase | 1 | PCAR0190 to PCAR0195 | Unknown | + | + |
| PCAR2867 | Cytochrome c nitrite reductase, nrfH | 4 | PCAR2866 | Nitrite reduction, electron transfer | + | + |
| PCAR2866 | Cytochrome c nitrite reductase, nrfA | 5 | PCAR2867 | Nitrite reduction, catalytic subunit | + | + |
| PCAR2550 | Cytochrome c | 5 | PCAR2549 to PCAR2553 | Electron transfer from quinol:cytochrome c oxidoreductase | − | + |
| PCAR1628 | Cytochrome c7, ppcA | 3 | None | Periplasmic electron carrier | − | + |
| Outer membrane associated | ||||||
| PCAR0152 | Hypothetical protein | 1 | PCAR0153 to PCAR0159 | Associated with Fe3+-siderophore ABC transporter | + | − |
| PCAR2984 | Cytochrome c family protein | 2 | PCAR2983 | Associated with pyruvate kinase | + | + |
| PCAR2745 | Hypothetical protein | 3 | None | Unknown | + | + |
| PCAR2069 | Protein disulfide isomerase | 1 | None | Unknown | + | + |