FIG. 4.

Least-squares distance tree of archaeal 16S rRNA gene and rRNA clones from GC205 sediment depths SURF (0 to 3 cm), SMI-1 (15 to 18 cm), and SMI-2 (18 to 21 cm) (boldface). The bootstrap values, >99% similarity groupings, distance scale, and environmental-clone names follow the conventions in Fig. 3. For SMI-1 the >99% similar clone numbers are listed for each reverse primer used. Sequences including “RNA” in their names are cDNAs that were reverse transcribed from total RNA; all other sequences were PCR amplified from DNA. TMEG, terrestrial miscellaneous euryarchaeotal group (61); DSAG, deep-sea archaeal group. See the text for explanations of other phylogenetic group acronyms.