Numbers of basidiomycete “species” detected in soil samples from KBS-LTER in June and October 2002. A. Number of “species” per sample (with “species” defined as having ≥99% sequence identity over the 5′ nLSU rDNA region), with samples arranged in order from those with fewest to most “species” (65 samples yielded no basidiomycetes [not shown]). B. Species accumulation curve for the combined samples from all replicates and treatments sampled (conventional tillage, no-till, historically tilled successional community, and never-tilled successional community). Points on the “species” accumulation curve represent the sample-based rarefaction curve derived using the Mau Tau algorithm in EstimateS (version 7.5; Robert K. Colwell, Department of Ecology and Evolutionary Biology, University of Connecticut [http://purl.oclc.org/estimates]), and the error bars depict the 95% confidence interval of the estimate.