Effects of temperature on the conversion of E-type and C-type strains. Two E-type strains, JY1305 and Env1 (shaded bars), and two C-type strains, C7184K and CMCP6 (solid bars), were compared for population conversion, with each strain having 15 replicate assays performed. Increasing temperature was used under conditions described in the legend of Fig. 1. Statistical analysis was performed by using two-way ANOVA followed by a Bonferroni posttest. Strains, temperature, and their interactions were all found to be statistically significant (P < 0.0001 for all).