TABLE 2.
Oligonucleotides used in this study
| Primer | Sequence and restriction sitesa | Description |
|---|---|---|
| Bac43-1 | GAATTCAAAACTACTTTTTATGACG | Analysis of bac determinant |
| Bac43-2 | GAATTCTAGGAACTTGTCTAGCTGG | |
| Bac43-5′ | GAATTCTATGATAATTTTTCGGCTC | |
| Bac43-4′ | GAATTCGATAGTCATCTATAGTTGC | |
| Bac43-6′ | GAATTCAAGCCCATCCTCTATATAC | |
| Tn5 | CAGATTTAGCCCAGTCGG | Analysis of Tn5 insetion mutant |
| J1 | GAGTATTGCAACTTGCTCGC | Analysis of EcoRI junction of pTD1 |
| J2 | GCTACAAGAAGTGGTTCGGC | |
| C | TTGGTACAGGCGTTACTTGG | Analysis of bacA gene |
| E2 | ATCCGAATTCATAACCTCCCTACCACTACC | |
| H1 | CGAAAAGGAAAAACAATCATG | Analysis of bac43 determinants |
| H2 | TCCCATTTTCATTTTATTCC | |
| M1 | AAGGGTGGGACTTATGAGCG | Analysis of mob genes |
| M2 | TTGTTGGTAGTCTGCTCCTC |
a
Underlined letters indicate restriction sites (GAATTC; EcoRI).