TABLE 3.
Genetic features and occurrence of recombination at the five MLST loci
Locus | Data seta | No. of alleles | % of VIb | Nucleotide diversity per site | G+C content (%) | Ka/Ks | Recombination (MaxChi, P < 0.01)c |
---|---|---|---|---|---|---|---|
gatB | ABDF | 26 | 28.7 | 0.078 | 37.2 | 0.093 | Yes (G. firmus)d |
A | 13 | 9.2 | 0.027 | 37.8 | 0.151 | No | |
B | 11 | 11.1 | 0.022 | 36.6 | 0.135 | No | |
coxA | ABDF | 27 | 28.4 | 0.083 | 39.3 | 0.078 | No |
A | 11 | 4.5 | 0.015 | 39.9 | 0.099 | No | |
B | 14 | 17.4 | 0.053 | 38.7 | 0.072 | Yes (P. sialia[B1], T. confusum, E. formosa) | |
hcpA | ABDF | 31 | 36.5 | 0.081 | 37.0 | 0.140 | No |
A | 13 | 12.6 | 0.029 | 37.7 | 0.090 | Yes (A. albopictus, A. sparsa) | |
B | 16 | 15.6 | 0.028 | 36.3 | 0.346 | No | |
fbpA | ABDF | 28 | 30.5 | 0.092 | 38.6 | 0.084 | Yes (A. vulgare) |
A | 12 | 9.0 | 0.034 | 38.7 | 0.085 | No | |
B | 15 | 20.1 | 0.063 | 38.7 | 0.089 | Yes (C. alternans) | |
ftsZ | ABDF | 25 | 24.8 | 0.065 | 41.5 | 0.047 | No |
A | 10 | 6.4 | 0.012 | 42.6 | 0.028 | No | |
B | 14 | 10.3 | 0.020 | 40.4 | 0.065 | No |
Data sets A and B included 18 supergroup A strains and 17 supergroup B strains, respectively (Table 2).
The percentage of variable sites was estimated by considering indels in each data set variable sites.
Recombination inferences for data set ABDF refer to events that occurred between supergroups.
The organisms in parentheses are hosts of putative recombinant strains.