TABLE 2.

Oligonucleotides used in this study^a

Primer	Sequence
SSS_UP	5′ CGT CTT TCG CAC CTG ATG GA 3′
SSS_LOW	5′ CCT GGG ATG GGC ACT GTC A 3′
ORFT_UP	5′ CTA CGT TTA CAA CAG CTG GA 3′
ORFT_LOW	5′ TTC ACC CAG TTC GCT CAG T 3′
ORFT_KpnI	5′ AAG TAG TAC GGG GTG ACC AG 3′
ORFT_Bam1	5′ TTT TGG ATC CAC GAA GGG TTT GCA 3′
ORFT_Bam2	5′ GCT GGA TCC TCA GGA TGC GGT C 3′
ORFT_Pst	5′ GCC CCA GCA ATA CAA ACA AC 3′
ORFT_seq1	5′ GAC GAT TTG TTC CGC GAT GC 3′
ORFT_seq2	5′ TCC AGC CAG CCG CGC ACA C 3′
PTSP3	5′ TCCAACGACCTCACCCAGTA 3′
PTSP4	5′ CGCAGCATCCACTTCACCTT 3′
PTSP5	5′ GGT GGT GAC ATT CAA GCG CGA 3′
PTSP13	5′ GAG TGT GTT CAT CGC CAG CC 3′
KAN_UP	5′ TGG CAA GAT CCT GGT ATC GGT 3′
KAN_LOW	5′ GAA ACA TGG CAA AGG TAG CGT 3′
Cm_UP	5′ ATC CCA ATG GCA TCG TAA AGA 3
Cm_LOW	5′ AAG CAT TCT GCC GAC AT 3′
sssF	5′ GTG CCC TTT GTC AGC CTG ATC CTG TTC G 3′
sssR	5′ AGC TGG GTT CTA TGA TTC GTC GCC CTT GAT GC 3′
sss2F	5′ GGG GAC AAG TTT GTA CAA AAA AGC AGG CTT AGT GCC CTT TGT CAG C 3′
ptsPF	5′ GGA GGC TCT TCA ATG CTC AAT ACG CTG CGC AA 3′
ptsPR	5′ AGC TGG GTT CTA GAG GGT CTT GTT CGA AGC CG 3′
orfTF	5′ GGA GGC TCT TCA ATG CCC GAC CAA GAT GTA CG 3′
orfTR	5′ AGC TGG GTT CTA TGC CGT TGC TCC GGC CGA TT 3′

^aOligonucleotides were designed by using the Oligo 6.65 primer analysis software and were constructed in this study.