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. 2006 Aug 25;72(11):7365–7367. doi: 10.1128/AEM.00889-06

FIG. 2.

FIG. 2.

(A) Eckhardt gel displaying plasmid profiles of strains WSM1271, WSM1283, WSM1284, WSM1497, N17, N18, N45, and N87; M. loti strain ICMP3153 (negative control); and Rhizobium leguminosarum strain VF39 (positive control used to estimate the approximate sizes of plasmids). (B) Southern blot of the Eckhardt gel hybridized with a probe mixture consisting of nifH and nodA of Biserrula mesorhizobia and nodC of R. leguminosarum. Note the hybridization of the nodC probe to the 240-kb plasmid of strain VF39 and the hybridization of the nifH and nodA probes to the chromosomal DNA in the wells.