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Synthesis of oligosaccharides using immobilized glycosyltransferases. (a) Synthesis of Lewis x, Lewis y, H antigen, A antigen, and B antigen from LNnT-PA using immobilized FUT1, FUT6, and A(ABO) or B(ABO). (b) Synthesis of GM1b and GD1α from asialo-GM1 tetraose-PA using immobilized ST3Gal I and ST6GalNAc IV. (c) Synthesis of fucosylated and branched N-glycans using FUT8, MGAT4A, and MGAT5.
