TABLE 2.
Effects of Pir anchor species on the enzymatic activities of various glycosyltransferases
| Gene | Host | Anchor(s) | Acceptor | Activity (arbitrary units) |
|---|---|---|---|---|
| ST3Gal I | Δpir1,2,3 | PIR1 | Asialo-GM1-tetrasaccharide-PA | 3,917 |
| PIR3 | Asialo-GM1-tetrasaccharide-PA | 4,608 | ||
| PIR4 | Asialo-GM1-tetrasaccharide-PA | 4,032 | ||
| ST3Gal IV | Δpir1,2,3 | PIR1 | LNnT-PA | 0 |
| PIR3 | LNnT-PA | 8 | ||
| PIR1, PIR3 | LNnT-PA | 30 | ||
| ST6Gal I | Δpir1,2,3 | PIR1 | LNnT-PA | 0 |
| PIR3 | LNnT-PA | 70 | ||
| ST6Gal II | Δpir1,2,3 | PIR1 | LNnT-PA | 0 |
| PIR3 | LNnT-PA | 3 | ||
| ST6GalNAc IV | Δpir1,2,3 | PIR1 | NeuAcα2,3Galβ1,3GalNAcβ1,4Galβ1,4Glc-PA (ST3Gal I product) | 11 |
| FUT1 | Δpir1,2,3 | PIR1 | LNnT-PA | 0 |
| LNT-PA | 0 | |||
| PIR3 | LNnT-PA | 48 | ||
| LNT-PA | 74 | |||
| FUT3 | Δpir1,2,3 | PIR1 | LNT-PA | 0 |
| PIR3 | LNT-PA | 16 | ||
| FUT8 | Δpir1,2,3 | PIR3 | N-Biantennary-Asn-PABA | >100 |