TABLE 3.
Glycosyltransferase assays of various pp-GalNAc-T's
| Gene | Host | Anchor | Acceptor | Activity (arbitrary units) |
|---|---|---|---|---|
| pp-GalNAc-T1 | Δpir1,2,4 PDI1 | PIR4 | FITC-Muc5AC | >500 |
| pp-GalNAc-T2 | Δpir1,2,4 PDI1 | PIR4 | FITC-Muc5AC | 475 |
| pp-GalNAc-T3 | Δpir1,2,3 PDI1 | PIR3 | FITC-Muc5AC | 0 |
| Δpir1,2,4 PDI1 | PIR4 | FITC-Muc5AC | 250 | |
| pp-GalNAc-T4 | Δpir1,2,3 PDI1 | PIR3 | FITC-Muc5AC | 0 |
| Δpir1,2,4 PDI1 | PIR4 | FITC-Muc5AC | 150 | |
| pp-GalNAc-T6 | Δpir1,2,3 | PIR1 | FITC-Muc5AC | 35 |
| Δpir1,2,3 PDI1 | PIR1 | FITC-Muc5AC | 258 | |
| pp-GalNAc-T7 | Δpir1,2,4 PDI1 | PIR4 | FITC-Muc5AC-GalNAc (pp-GalNAc-T1 product) | 0 |
| PIR1, PIR4 | FITC-Muc5AC-GalNAc (pp-GalNAc-T1 product) | 55 | ||
| pp-GalNAc-T8 | Δpir1,2,3 PDI1 | PIR1, PIR3 | FITC-Muc5AC | 0 |
| pp-GalNAc-T9 | Δpir1,2,3 PDI1 | PIR1 | FITC-Muc5AC-GalNAc (pp-GalNAc-T1 product) | 0 |
| pp-GalNAc-T10 | Δpir1,2,4 | PIR4 | FITC-Muc5AC | 90 |
| Δpir1,2,4 PDI1 | PIR4 | FITC-Muc5AC | 215 | |
| pp-GalNAc-T12 | Δpir1,2,4 PDI1 | PIR4 | FITC-Muc5AC | 95 |
| pp-GalNAc-T13 | Δpir1,2,3 | PIR1 | FITC-Muc5AC | 485 |
| pp-GalNAc-T14 | Δpir1,2,3 PD1I | PIR1 | FITC-Muc5AC | 0 |
| pp-GalNAc-T15 | Δpir1,2,4 PDI1 | PIR4 | FITC-Muc5AC | 0 |