FIG. 3.

(A) Resorcinol hydroxylase activity in recombinant E. coli/pET28a-NCgl1111. (B) E. coli/pET28a was used as a negative control. The absorbances of the reaction mixture at wavelengths of 270 to 400 nm were recorded at time zero (top line) and at 60, 120, and 180 s (bottom line), Hydroxylase activity was also determined with 3-hydroxybenzoate, 3-aminophenol, 3,5-dihydroxytoluene, and 2,4-dihydroxybenzoate, but no activity was detected.