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. 2006 Aug 25;72(11):6886–6893. doi: 10.1128/AEM.00868-06

FIG. 2.

FIG. 2.

ML tree based on a 2,075-bp region of the viral envelope glycoprotein genes for 32 Nebraska BCRV isolates from 2001, a Colorado isolate from 2001 (labeled COL 2001), 6 FMV isolates collected from 1974 to 1975 (75V7468, 77V654, CM4-110, CM4-146, CM4-368, and CM4-976), and 6 Oklahoma BCRV isolates collected from 1981 to 1983 (81V1822, 81V1823, 81V1824, 81V1825, 81V1826, and 84S217). A 1971 WEEV sequence (71V1658 [GenBank accession number AF214040]) was used as an outgroup to root the tree (not shown). GenBank accession numbers are given in Materials and Methods. Nebraskan BCRV subclades are labeled NebA-N1, NebA-N2, NebA-N3, NebB-N1, NebB-N2, and NebB-N3; the Oklahoma BCRV clade is labeled Okl; the Colorado clade is labeled Col; and the Colorado subclade is labeled NebA-C (see Results). Different virus clades and subclades are color coded for illustrative purposes. Bootstrap support was generated using 1,000 replicate neighbor-joining trees reconstructed under the ML substitution model (27).