TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| E. coli | ||
| BW20767 | Pir+mob+ | S. Minnoch, University of Idaho |
| JM109 | recA endA | Promega, Madison, WI |
| S17.1 | Smrmob+ | S. Minnoch |
| P. stutzeri | ||
| KC | Wild-type PDTC producer | C. Criddle, Stanford University |
| SEMΔpdtC | KC ΔpdtC::Km | This study |
| P. putida | ||
| KT2440 | Wild type | Estacion Exp. Zaidin (EEZ), Granada, Spain |
| PSC 48 | KT2440 rpoS null mutant | EEZ (33) |
| PSC 39 | KT2440 rpoN null mutant | EEZ (18) |
| Plasmids | ||
| pEP185.2sacB1 | Cmrtra+oriR6KsacB vehicles for allele replacement and counterselection | 23 |
| pEP185.2sacB1Δsal | pEP185.2sacB1 lacking SalI-XhoI of cloning site | This study |
| pSEMΔC | pEP185.2sacB1Δsal with ΔpdtC::Km allele | This study |
| pGEM T-easy | AproricolE1cloning vector | Promega |
| pJB3Tc20 | Tcr RK2-based IncP broad-host-range vector | 6 |
| PSEMC | pJB3Tc20 with pdtC gene inserted between KpnI and SphI sites | This study |
| pMP77 | Strr IncQ broad-host-range promoter-probe vector with promoterless xylE | 39 |
| pFpro1 (etc.) | Series of constructs: pMP77 with pdtF promoter fragments of various lengths inserted upstream of xylE using BglII and HindIII sites (Fig. 4) | This study |
| pCpro | pMP77 with pdtC promoter fragment inserted upstream of xylE using BglII and HindIII sites | This study |