TABLE 2.
Primers used in this study
| Primer name | Sequencea | Target | Positionb |
|---|---|---|---|
| pdtCLl2 | ATCTCGAGGTTGCCTGCCCTTGACTG | pdtC downstream flanking region | 1972-1990 (+) |
| pdtCLr | ATGAGCTCGTCGACCGCTCCAGCCTAATGTACTG | pdtC downstream flanking region | 3301-3320 (−) |
| pdtCRl | ATCAGTCGACGTGATGACCATGACCCGAC | pdtC upstream flanking region | 4101-4118 (+) |
| pdtCRr2 | GAAGATCTCCAATCAGCAGTCCGACC | pdtC upstream flanking region | 5573-5590 (−) |
| pdtCpro4066Xba | TCTAGACCGTCGTAGCAGGAG | pdtC promoter | 4066-4080 (+) |
| pdtCpro4502Bgl | AGATCTCGGGCGTGCAGTGC | pdtC promoter | 4502-4518 (−) |
| pdtC3958 | GCGAAACCGTAACGTCCG | pdtC cDNA (RACE) | 3958-3975 (+) |
| pdtC3803 | GGCTGATCAACGCGTTG | pdtC cDNA (RACE) | 3808-3824 (+) |
| pdtCrev3391 | GCGATTTGCCGTGGTAGCGAGTAA | pdtC mRNA | 3391-3414 (+) |
| pdtCfwd3638 | CAGGCACGGCAGATGATGGATGAG | pdtC cDNA | 3638-3661 (−) |
| pdtproFwd6184 | ATAGATCTACACCGGCATGC | pdtF promoter | 6184-6196 (+) |
| pdtproFwd6266 | ATAGATCTTCGGCACAGTCG | pdtF promoter | 6266-6278 (+) |
| pdtproFwd6302 | ATAGATCTAAGGCTGAAGTGACCGGC | pdtF promoter | 6294-6319 (+) |
| pdtproFwd6328 | GCCATAGATCTTCGGACAATG | pdtF promoter | 6328-6338 (+) |
| pdtproFwd6356 | GCGCAGATCTTTTGCATAG | pdtF promoter | 6356-6366 (+) |
| pdtproFwd6377 | TAGATCTCACGTCATATGAAAGAACAGCC | pdtF promoter | 6377-6399 (+) |
| pdtpro6400 | AAGATCTAACGGCAATTGCTATAG | pdtF promoter | 6400-6416 (+) |
| pdtproR | ATTCTAGACCTGTTTGATTTCAATGGTAACG | pdtF promoter | 6443-6465 (−) |
| pdtpro6410 | GATCTAGACAATTGCCGTTGGCTGTTC | pdtF promoter | 6392-6410 (−) |
| pdtFFwd | CGGTGTGTTCGGCATTCTTT | pdtF cDNA | 7086-7105 (+) |
| pdtFRev | CCCGATAGCGCATATCCAAA | pdtF mRNA | 7200-7219 (−) |
| AlMoeZR | ACGGGTTGCGAAGTTGTC | pdtF mRNA | 6889-6906 (−) |
| pdtEfwd | CGTTCCGCAGCGCTGTTCGTAG | pdtE cDNA | 5475-5496 (+) |
| pdtErev | CGCCCGGTGGAGGATCTGTAGTCT | pdtE mRNA | 5653-5676 (−) |
| pdtP22937 | CTGGCGCCAATGGAAACAAGTCG | pdtP mRNA | 22937-22959 (+) |
| pdtP23114 | GGGCGGGGTTTTCGTGAGCA | pdtP cDNA | 23114-23133 (−) |
| KC16sFwd | GCGGTGGAGCATGTGGTT | 16S cDNA | |
| KC16sRev | CTGGAAAGTTCTCTGCATGTCAA | 16S rRNA | |
| Abridged anchor | GGCCACGCGTCGACTAGTACGGGIIGGGIIGGGIIG | Poly(C)-tailed cDNA | |
| Abridged universal | GGCCACGCGTCGACTAGTAC | Amplified cDNA |
a
Boldface letters indicate restriction sites.
b
GenBank accession number AF196567.