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Reconstitution of pdtF promoter expression in the heterologous host P. putida KT2440 by pdtC (in trans) plus cognate siderophore
| Culturea | XylE activity (nmol min−1 mg−1)b |
|---|---|
| KT2440 | ND |
| KT2440/pFpro3 | ND |
| KT2440/pFpro3 + PDTCc | ND |
| KT2440/pFpro3 pSEMC | ND |
| KT2440/pFpro3 pSEMC + PDTC | 7.2 ± 1.8 |
Cultures were grown in PM glucose/glutamine.
Mean ± standard error of three independent experiments. ND, not detected.
PDTC was added to 20 μM.
