TABLE 2.
Oligonucleotides used in this study
| Oligonucleotide | Sequence (5′→3′)a | Restriction site |
|---|---|---|
| SF-yscU1 | CCATCGATGGCGATCGCTTCTCATGTTGTGCA | ClaI |
| SF-yscU2 | GCTCTAGAGCAATATGGGTCGGATTAGCTACC | XbaI |
| SF-orf76-1 | GCTCTAGATGAGCCAGCTTCCAGAACACG | XbaI |
| SF-orf76-2 | GAAGATCTAAAACGATTCGCCCCTGAAAG | BglII |
| SF-orf83-1 | GCTCTAGATTGCTCGCGAATAACACTCAC | XbaI |
| SF-orf83-2 | GAAGATCTATTGGTCGCCAGCTGATAAAA | BglII |
| SF-yscN1 | GCTCTAGACTGGAACTTTGCTAAGGTATT | XbaI |
| SF-yscN2 | GAAGATCTTTGAGTGACACGTCCTCTAAT | BglII |
| SF-lcrGRT1 | CAAGCAGAACTGGCAATAGC | |
| SF-lcrGRT2 | CCTCGCATCATCGTTGGTTT | |
| SF-lcrG3 | GCTCTAGACACTTGGACGAGTTTGCTTAT | XbaI |
| SF-lcrG4 | GAAGATCTTAATTTTGCGCGGTGATCGCT | BglII |
| SF-yopNRT1 | GACGTTGAGGAGCAGGTTAA | |
| SF-yopNRT2 | ATAACCCATCACTGCATCGC | |
| SF-yopN3 | GCTCTAGACAGTATTAGAAGATATCCCGT | XbaI |
| SF-yopN4 | GAAGATCTATCAGCTATAGACTGCAGAGT | BglII |
| SF-lonRT1 | GCCATTCAGAAAGAACTGGGC | |
| SF-lonRT2 | GGCACCTGCAACATCCAATC | |
| SF-lon5 | GAAGATCTTACCCGGCATAGAGCCAATA | BglII |
| SF-clpPRT1 | TTTTCCTGACAGGCCAGGGTTG | |
| SF-clpPRT2 | ATGCACCCATTGAACATGCC | |
| SF-clpP5 | GAAGATCTTGCCATCAGTTCATTCATGC | BglII |
| SF-yhbURT1 | CAGCCGCAAACGGAAATTG | |
| SF-yhbURT2 | TTCATCATTGGTCGCCGAGG | |
| SF-rnaYE1 | AATACCGCATAACGTCTTCG | |
| SF-rnaYE2 | CTTCTTCTGCGAGTAACGTC | |
| SF-lcrG5 | CCGCTCGAGTTAATATCCAACCACTTGGAC | XhoI |
| SF-lcrG6 | GAAGATCTAATCTATCTCCTATTCTATTA | BglII |
| SF-lcrG7 | GAAGATCTTATGATTAGAGCCTACGAACA | BglII |
| SF-lcrG8 | TCCCCGCGGGTAAGCTCAGCTAATTCTTCA | SacII |
a
The restriction site is underlined.