TABLE 2.
Oligonucleotide | Sequence (5′→3′)a | Restriction site |
---|---|---|
SF-yscU1 | CCATCGATGGCGATCGCTTCTCATGTTGTGCA | ClaI |
SF-yscU2 | GCTCTAGAGCAATATGGGTCGGATTAGCTACC | XbaI |
SF-orf76-1 | GCTCTAGATGAGCCAGCTTCCAGAACACG | XbaI |
SF-orf76-2 | GAAGATCTAAAACGATTCGCCCCTGAAAG | BglII |
SF-orf83-1 | GCTCTAGATTGCTCGCGAATAACACTCAC | XbaI |
SF-orf83-2 | GAAGATCTATTGGTCGCCAGCTGATAAAA | BglII |
SF-yscN1 | GCTCTAGACTGGAACTTTGCTAAGGTATT | XbaI |
SF-yscN2 | GAAGATCTTTGAGTGACACGTCCTCTAAT | BglII |
SF-lcrGRT1 | CAAGCAGAACTGGCAATAGC | |
SF-lcrGRT2 | CCTCGCATCATCGTTGGTTT | |
SF-lcrG3 | GCTCTAGACACTTGGACGAGTTTGCTTAT | XbaI |
SF-lcrG4 | GAAGATCTTAATTTTGCGCGGTGATCGCT | BglII |
SF-yopNRT1 | GACGTTGAGGAGCAGGTTAA | |
SF-yopNRT2 | ATAACCCATCACTGCATCGC | |
SF-yopN3 | GCTCTAGACAGTATTAGAAGATATCCCGT | XbaI |
SF-yopN4 | GAAGATCTATCAGCTATAGACTGCAGAGT | BglII |
SF-lonRT1 | GCCATTCAGAAAGAACTGGGC | |
SF-lonRT2 | GGCACCTGCAACATCCAATC | |
SF-lon5 | GAAGATCTTACCCGGCATAGAGCCAATA | BglII |
SF-clpPRT1 | TTTTCCTGACAGGCCAGGGTTG | |
SF-clpPRT2 | ATGCACCCATTGAACATGCC | |
SF-clpP5 | GAAGATCTTGCCATCAGTTCATTCATGC | BglII |
SF-yhbURT1 | CAGCCGCAAACGGAAATTG | |
SF-yhbURT2 | TTCATCATTGGTCGCCGAGG | |
SF-rnaYE1 | AATACCGCATAACGTCTTCG | |
SF-rnaYE2 | CTTCTTCTGCGAGTAACGTC | |
SF-lcrG5 | CCGCTCGAGTTAATATCCAACCACTTGGAC | XhoI |
SF-lcrG6 | GAAGATCTAATCTATCTCCTATTCTATTA | BglII |
SF-lcrG7 | GAAGATCTTATGATTAGAGCCTACGAACA | BglII |
SF-lcrG8 | TCCCCGCGGGTAAGCTCAGCTAATTCTTCA | SacII |
The restriction site is underlined.