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. 2006 Oct;188(20):7132–7140. doi: 10.1128/JB.00647-06

FIG. 2.

FIG. 2.

Expression of FtsZ-CtYFP in the suppressor strain. (A) The original strain JKD7-1 and the suppressor strain generated from FtsZ-CtYFP were compared by immunoblotting, as described previously (45). One microgram of total protein from the suppressor strain and 2, 1, and 0.5 μg from JKD7-1 were loaded. The cells were cultured with 0.2% arabinose at 42°C for the suppressor strain and at 30°C for JKD7-1. (B) Fluorescence microscopy localizing FtsZ-CtYFP in the suppressor strain SUP{CtYFP1}, where it is the sole source of FtsZ.