TABLE 1.
Strains and plasmids
| Strain or plasmid | Descriptiona | Reference |
|---|---|---|
| Strains | ||
| JKD7-1 | ftsZ::Kanr (genomic ftsZ null) recA | 7 |
| JSN2 | JKD7-1/pKD3/pJSB2 | 45 |
| wt | JKD7-1/pJSB2-EcFtsZ | 45 |
| SUP{D96A}-D96A | JKD7-1/pJSB2-D96A (mut?) | This study |
| SUP{D96A}-wt | JKD7-1/pKD3/pBAD18Spcr (mut?) | This study |
| SUP{Q47K}-Q47K | JKD7-1/pJSB2-Q47K (mut?) | This study |
| SUP{Q47K}-wt | JKD7-1/pKD3/pBAD18Spcr (mut?) | This study |
| SUP{D212G1}-D212G | JKD7-1/pJSB2-D212G (mut?) | This study |
| SUP{D212G1}-wt | JKD7-1/pKD3/pBAD18Spcr (mut?) | This study |
| SUP{D212G2}-D212G | JKD7-1/pJSB2-D212G (mut?) | This study |
| SUP{D212G2}-wt | JKD7-1/pKD3/pBAD18Spcr (mut?) | This study |
| SUP{CtYFP1}-CtYFP1 | JKD7-1/pJSB2-CtYFP (mut?) | This study |
| SUP{CtYFP1}-wt | JKD7-1/pKD3/pBAD18Spcr (mut?) | This study |
| SUP{CtYFP2}-CtYFP1 | JKD7-1/pJSB2-CtYFP (mut?) | This study |
| SUP{CtYFP2}-wt | JKD7-1/pKD3/pBAD18Spcr (mut?) | This study |
| SUP{326YFP}-326YFP1 | JKD7-1/pJSB2-326YFP (mut?) | This study |
| SUP{326YFP}-wt | JKD7-1/pKD3/pBAD18Spcr (mut?) | This study |
| SUP{CtYFP1//D96A}-D96A | JKD7-1/pJSB2-D96A (mut? mut?) | This study |
| SUP{CtYFP1//D96A}-wt | JKD7-1/pKD3/pBAD18Spcr (mut? mut?) | This study |
| SUP{CtYFP1//Q47K}-Q47K | JKD7-1/pJSB2-D96A (mut? mut?) | This study |
| SUP{CtYFP1//Q47K}-wt | JKD7-1/pKD3/pBAD18Spcr (mut? mut?) | This study |
| SUP{D212G1//CtYFP}-CtYFP | JKD7-1/pJSB2-CtYFP (mut? mut?) | This study |
| SUP{D212G1//CtYFP}-wt | JKD7-1/pKD3/pBAD18Spcr (mut? mut?) | This study |
| SUP{Av}-Av | JKD7-1/pJSB2-AvftsZ (mut?) | This study |
| SUP{Pa}-Pa | JKD7-1/pJSB2-PaftsZ (mut?) | This study |
| SUP{Bs/EcCt}-Bs/EcCt | JKD7-1/pJSB2-Bs/EcCt ftsZ (mut?) | This study |
| SUP{Mpu/EcCt11}-Mpu/EcCt | JKD7-1/pJSB2-Mpu/EcCt ftsZ (mut?) | This study |
| SUP{Mpu/EcCt11}-wt | JKD7-1/pKD3/pBAD18Spcr (mut?) | This study |
| SUP{Mpu/EcCt13}-Mpu/EcCt | JKD7-1/pJSB2-Mpu/EcCt ftsZ (mut?) | This study |
| SUP{Mpu/EcCt13}-wt | JKD7-1/pKD3/pBAD18Spcr (mut?) | This study |
| SUP{Mpu/EcCt28}-Mpu/EcCt | JKD7-1/pJSB2-Mpu/EcCt ftsZ (mut?) | This study |
| SUP{Mpu/EcCt28}-wt | JKD7-1/pKD3/pBAD18Spcr (mut?) | This study |
| Plasmids | ||
| pKD3 | pSC101 origin; repA(Ts) ftsZ Ampr | 7 |
| pBAD18 | pBR322 origin; arabinose regulation; Ampr | 22 |
| pBAD18Spcr | pBAD18-bla::aadA (Amps Spcr) | This study |
| pJSB1 | pBAD18 with MCSa for blunt insertion | 45 |
| pJSB2 | pJSB1-bla::cat (Amps Cmr) | 45 |
| pJSB2-wt | pJSB2-EcftsZ | 45 |
| pJSB2-Q47K | pJSB2-EcftsZ-Q47K | 43 |
| pJSB2-D96A | pJSB2-EcftsZ-D96A | 45 |
| pJSB2-D212G | pJSB2-EcftsZ-D212G | 45 |
| pJSB2-248-20aa | pJSB2-EcftsZ-248-20aa | 38 |
| pJSB2-326YFP | pJSB2-EcftsZ-326-YFP | 38 |
| pJSB2-348-20aa | pJSB2-EcftsZ-348-20aa | 38 |
| pJSB2-348YFP | pJSB2-EcftsZ-348YFP | 38 |
| pJSB2-CtYFP | pJSB2-EcftsZ-CtYFP | This study |
| pJSB2-Yp | pJSB2-YpftsZ | This study |
| pJSB2-Pa | pJSB2-PaftsZ | This study |
| pJSB2-Av | pJSB2-AvftsZ | This study |
| pJSB2-Bs | pJSB2-BsftsZ | This study |
| pJSB2-Bs/EcCt | pJSB2-Bs/EcCtftsZ | This study |
| pJSB2-Mpu | pJSB2-MpuftsZ | This study |
| pJSB2-Mpu/EcCt | pJSB2-Mpu/EcCtftsZ | This study |
| pJSB2-Mpn | pJSB2-MpnftsZ | This study |
| pJSB2-Mpn/EcCt | pJSB2-Mpn/EcCtftsZ | This study |
a
mut? indicates an unknown suppressor mutation in the genome; mut? mut? indicates unknown double suppressor mutations in the genome.