TABLE 3.
Foreign FtsZs tested for complementation and production of suppressor strainsa
| FtsZ strain | Complementation with foreign FtsZ from:
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| E. coli | Y. pestis | P. aeruginosa | A. vinlandii | B. subtilis | Bs/EcCt | M. pulmonis | Mpu/EcCt | M. pneumoniae | Mpn/EcCt | |
| Wild type | C | C | S (∼10−3) | S (10−4-10−5) | NS | S (10−4-10−5) | NS | S (10−4-10−5) | NS | NS |
| SUP{D96A} | C | C | NC | NC | NC | NC | NC | NS | ||
| SUP{Q47K} | C | C | NC | NC | NC | NC | NC | NC | ||
| SUP{D212G1} | C | C | C | C? (0.1) | C? (0.1) | C | NC | |||
| SUP{D212G2} | C | C | C | C? (0.1) | NC | C | NC | |||
| SUP{CtYFP1} | C | C | C | C | NS | C | NS | NC, NS | NS | NS |
| SUP{CtYFP2} | C | C | C | NC | C | NC | NC | |||
| SUP{CtYFP1//D96A} | C | C | C? (0.1) | NC | C | C | NC | |||
| SUP{CtYFP1//Q47K} | C | C | C | C | C | C? (0.1) | NC | |||
| SUP{D212G1//CtYFP} | C | C | C | C? (0.4) | C? (0.4) | C? (0.3) | NC | |||
| SUP{Mpu/EcCt13} | C | C | C | NC | C | C | NC | |||
a
Bs/EcCt, B. subtilis FtsZ with the C-terminal peptide from E. coli; Mpu/EcCt, M. pulmonis FtsZ with the C-terminal peptide from E. coli; Mpn/EcCt, M. pneumoniae FtsZ with the C-terminal peptide from E. coli. Wild-type results show the results when each foreign FtsZ was tested in the wild-type E. coli background. C, the foreign FtsZ complemented in our standard assay; S, it did not complement, but it produced suppressor strains at the indicated frequency; NS, the construct was tested under suppressor generation conditions, but none were obtained; C? (0.1), colonies were obtained under complementing conditions but at 1/10 the normal frequency.