FIG. 4.

Immunoblot analysis of CseA processing. (A) Wild-type S. coelicolor was grown for 16 h in NMMP liquid medium containing 2 mM MgCl₂ in the presence or absence of globomycin (150 μg ml⁻¹), an inhibitor of lipoprotein signal peptidase II; whole-cell extracts were prepared, and CseA was detected by immunoblotting. (B) The cseA deletion mutant (J2172) carrying pIJ6962 (wild-type [WT] cseA) or pIJ6967 (C37A mutant) was grown for 16 h in NMMP liquid medium containing 2 mM MgCl₂, and CseA was detected by immunoblotting.