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. 2006 Oct;188(20):7049–7061. doi: 10.1128/JB.00688-06

FIG. 1.

FIG. 1.

(A) Microcin E492 gene cluster from plasmid pJEM15. Open reading frames are represented by arrows. The restriction sites relevant for plasmid constructions are indicated. (B) Amino acid sequence of mature 84-residue MceA. The positions of the stop codons inserted to generate the C-terminally truncated MceA mutants are indicated (-16C, -11C, -6C, and -1C). The posttranslational modification of the C-terminal serine is symbolized by an asterisk. The original ABC signal sequence of preMceA and the signal sequence of PhoA73-MceA are shown underneath. The mutated amino acid in PhoA73-MceA (Q14) is underlined. The signalless construct (Δss-MceA) consists of a methionine residue followed by the mature portion of preMceA.