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. 2006 Oct;188(20):7049–7061. doi: 10.1128/JB.00688-06

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Copyright © 2006, American Society for Microbiology

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FIG. 6. — Bactericidal activity of endogenous full-length and C-terminally truncated MceA. (A and B) Serial dilutions of overnight cultures of DH5α with pBAD101 (vector), pAB13 (6H-MceA), pAB13-1C (6H-MceA-1C), pAB13-6C (6H-MceA-6C), pAB13-11C (6H-MceA-11C), or pAB13-16C (6H-MceA-16C) were spotted (5 μl) on LB plates without arabinose (A) or with 0.2% arabinose (B) and incubated overnight at 37°C. Results shown here are representative of three independent experiments. (C) Synthesis levels of full-length and C-terminally truncated MceA. Cultures of DH5α with pBAD101 (vector), pAB13 (6H-MceA), pAB13-1C (6H-MceA-1C), pAB13-6C (6H-MceA-6C), pAB13-11C (6H-MceA-11C), or pAB13-16C (6H-MceA-16C) were pulse-labeled as described in Materials and Methods. 6H-tagged proteins were purified and separated by SDS-PAGE. The position of full-length 6H-MceA is indicated by an arrow.