TABLE 1.
E. coli strains
| Strain | Genotype/description | Source/reference |
|---|---|---|
| DH5α | F−deoR recA1 endA1 hsdR17 supE44 λ−thi-1 gyrA96 relA1 | 26 |
| DH10B-T1 | F−mcrA Δ(mrr-hsdRMS-mcrBC) φ80dlacZΔM15 ΔlacX74 deoR recA1 endA1 araD139 Δ(ara-leu)7697 galU galK λ−rpsL nupG fhuA | Invitrogen |
| BL21 | F−ompT hsdS(rB− mB−) gal dcm | Amersham |
| MC4100 | F−araD139 relA1 thi rpsL150 flbB5301 Δ(argF-lac)U169 deoC1 ptsF25 rbsR | 6 |
| DB503 | MC4100 malE16-1 Δara714 | Lab collection |
| AB4 | DB503 manY::Tn10 | This study |
| DB512 | MC4100 malE18-1 Δara714 rpoA341 | Lab collection |
| AB1 | λ− Δ(tonB-trpB)873 his IN(rrnD-rrnE)1 rph-1 | W3110hisΔtrpAB in reference 59 |
| AB2 | AB1 trp+tonB+ | Lab collection |
| MC1000 | F−araD139 Δ(ara-leu)7697 Δ(lac)X74 rpsL150 galU galK thi | 7 |
| AD85 | MC1000 malFΔ3 phoAΔ(PvuII) phoR F′lacIQpro with pACYClacIQ | 15 |
| AD413 | AD85 prlA4 | 15 |
| DPE271ΔLPM | ΔmanXYZ::cat | 24 |
| AB3 | DB503 ΔmanXYZ::cat | This study |
| MG1655 | λ−ilvG rfb-50 rph-1 | 3 |
| AB5 | MG1655 manY::Tn10 | This study |
| JCB606 | E. coli RV Δnir dipZ | 9 |
| AB6 | JCB606 manY::Tn10 | This study |
| H1443 | MC4100 aroB | 27 |
| H1876 | H1443 fepA::Tn10 fiu::MudX cir | 27 |
| AB7 | DH10B-T1 with pAB4 and pAB7 | This study |
| SP850 | λ− e14−relA1 spoT1 ΔcyaA1400::Kn thi-1 | 53 |
| AB8 | DB503 ΔcyaA1400::Kn | This study |