TABLE 2.
Plasmids
| Plasmid | Description | Source/reference |
|---|---|---|
| pJEM15 | Microcin E492 genes (Fig. 1A) | 57 |
| pAB3 | pBR322 with NsiI site, Apr | This study |
| pAB4 | pAB3 with mceE mceJ mceI mceH mceG mceF | This study |
| pAB5 | pACYC184 with mceA mceB mceC mceD; Tcr | This study |
| pAB6 | pAB5 with BglII site | This study |
| pAB7 | pAB6 ΔmceA | This study |
| pAB8 | pAB6 ΔmceAB | This study |
| pBAD24 | Vector with pBR322 replication origin, PBAD promoter and araC; Apr | 25 |
| pGB2 | Vector with pSC101 replication origin; Spr | 8 |
| pBAD101 | PBAD promoter and araC in pGB2 | This study |
| pBAD24phoA73 | pBAD24 expressing PhoA73 | Lab collection |
| pAB9 | pBAD101 expressing PhoA-MceA | This study |
| pAB10 | pBAD101 expressing ABC-MceA | This study |
| pAB11 | pBAD101 expressing PhoA73-MceA | This study |
| pAB12 | pBAD101 expressing Δss-MceA | This study |
| pAB13 | pBAD24 expressing MceA with an N-terminal hexahistidine tag (6H-MceA) | This study |
| pAB10-1C, -6C, -11C, and -16C | pAB10 expressing ABC-MceA without 1, 6, 11, and 16 C-terminal residues | This study |
| pAB13-1C, -6C, -11C, and -16C | pAB13 expressing 6H-MceA without 1, 6, 11, and 16 C-terminal residues | This study |
| pAB15 | pAB11 expressing TrxA fused to the C terminus of PhoA73-MceA | This study |
| pAB16 | pAB12 expressing TrxA fused to the C terminus of Δss-MceA | This study |
| pJFL | manX driven by Ptac; Apr | 52 |
| pTSP11 | manY driven by Ptac; Apr | 20 |
| pJFP-H6M | manYZ driven by Ptac; Apr | 21 |
| pJFLPM | manXYZ driven by Ptac; Apr | 21 |
| pBAD33 | Vector with pACYC184 replication origin, PBAD and araC; Cmr | 25 |
| pAB17 | pBAD33 with manZ driven by PBAD | This study |