TABLE 3.
MccE492 sensitivities of mannose permease-deficient strains and other strainsa
| Strain | Relevant genotype | Activity ± SD (AU/ml)b |
|---|---|---|
| DB503 | Wild type | 960 ± 111 |
| MG1655 | Wild type | 939 ± 148 |
| JCB606 | Wild type | 896 ± 222 |
| AB4 | manY::Tn10 | No activity |
| AB5 | manY::Tn10 | No activity |
| AB6 | manY::Tn10 | No activity |
| AB3 | ΔmanXYZ | No activity |
| AB3(pJFL) | ΔmanXYZ/manX+ | No activity |
| AB3(pTSP11) | ΔmanXYZ/manY+ | No activity |
| AB3(pAB17) | ΔmanXYZ/manZ+ | No activity |
| AB3(pJFP-H6M) | ΔmanXYZ/manYZ+ | 1045 ± 225 |
| AB3(pJFLPM) | ΔmanXYZ/manXYZ+ | 896 ± 222 |
| MG1655(pAB8) | Wild type | 939 ± 148 |
| MG1655(pAB7) | mceB+ | No activity |
| H1443 | Wild type | 960 ± 111 |
| H1876 | fepA fiu cir | No activity |
| AB2 | Wild type | 1045 ± 225 |
| AB1 | tonB | 48 ± 16 |
a
The bactericidal activity of purified MccE492 (10 mg/ml) was assessed in triplicate for the indicated strains by the critical dilution method (see Materials and Methods). Expression from the indicated plasmids was induced with 600 μM IPTG or 0.2% arabinose for Ptac or PBAD promoters, respectively.
b
AU, arbitrary units.