FIG. 3.

Predicted and actual mRNA transcripts for speB, prsA, and spy2040. (A) A schematic diagram of spy2040, speB, and prsA genes and their surroundings on the GAS SF370 genome. The predicted transcription initiation and termination sites are shown together with the predicted mRNA sizes. The directions of transcription are indicated by arrows. The relative intensities of the resultant transcripts obtained by Northern analysis are indicated by arrows of different thicknesses. (B) Northern analysis of a single blot hybridized with either spy2040, speB, or prsA probes with stripping of the blot between experiments. The calculated molecular masses of the bands are indicated on the left. (C) Primer extension analyses confirmed the transcription start site of prsA.