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Immunodetection of Bradyrhizobium japonicum NADP-dependent isocitrate dehydrogenase. Soluble protein extracts from isolated B. japonicum USDA110 and strain 5051 were subjected to separation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, transferred to membranes, and probed with rabbit polyclonal antibodies raised against B. japonicum NADP-dependent isocitrate dehydrogenase protein.
